Peptide/Protein Characterization
The Genomics Shared Service has a variety of instrumentation for the analysis and characterization of peptides, proteins and a wide range of other molecules. Included are preparative HPLC's, analytical HPLC's with UV/Vis, diode array, fluorescence and electrochemical detectors, three capillary electrophoresis units with UV/Vis, diode array, and laser-induced fluorescence detectors, and a MALDI-TOF mass spectrometer.
HPLC Applications Include:
- Reversed phase (analytical and preparative)
- Ion-pairing (analytical)
- Size exclusion (analytical and preparative)
- Ion exchange (analytical and preparative)
- Affinity interaction (analytical)
Modes of Capillary Electrophoresis Separations Available Include:
- Free solution
- Micellar electrokinetic chromatography (MEKC)
- Size exclusion
- Chiral
- Isoelectric focusing
These tools have been used to follow chemical, enzymatic, or metabolic reactions, inter- and intra-molecular binding, and stability studies.
Patricia Campbell, PhD, an analytical biochemist with more than 25 years of experience in the practice and training of HPLC and capillary electrophoresis techniques is responsible for these services. As many of these services involve custom design and methods development, fees are quoted upon request.
